| Product Name |
Reactive Group (X) (application) |
MW |
| SUNBRIGHT® OE-020CS |
-CO-CH2CH2-COO-NHS |
2,000 |
| SUNBRIGHT® OE-040CS |
4,000 |
| SUNBRIGHT® OE-080CS |
8,000 |
| BAMCOAT® DI-035G |
Cell Immobilization, cell culture, miroarray etc., |
BAMCOAT® DI-035G
- New technology for cell immobilizing systems without cell damage.
- Easy-to-use pre-coated glass bottom dishes.
- Available for numerous applications for observation of non-adherent cell in culture.
| ex. |
Observation of intercellular calcium
Observation of Green Fluorescence Protein (GFP) localization
Observation of mitosis (time-lapse observation) |
|
 |
|
Procedure for cell immobilization using BAMCOAT®
Comparison of cell immobilization between BAM and PLL-modified surfaces
Direct observation of intracellular calcium in situ
- Apply the treated cell suspension (50ul) by Fura-3(4uM).
- Wash with PBS to remove non-immobilized cells.
- After 10 minutes, apply the calcium ionophore, A23187(50uM,100ul), to the immobilized cells.
Observation of intracellular calcium in K562 cell line
| Optical microscope |
Fluorescence microscope
|
Calcium Ionophore |
 |
Before |
 |
After |
(Data from Prof. Nagamune, Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan).
